Figure 8. Bioluminescent response of PCL-2 to H₂O₂ in FVB-luc⁺ mice.

(a) Representative image (10 min post-injection) for mice injected with a mixture of PCL-2 and D-cysteine (IP, 0.05 μmol each, in 50 μL of 1:1 DMSO:PBS) immediately prior to injection of H₂O₂ (IP, 0, 0.5, 1.5, or 4.5 μmol, left to right, in 100 μL of PBS). (b) Total photon flux, 0–15 min post-injection, for mice injected with PCL-2 and D-cysteine ± H₂O₂. (c) Representative image (10 min post-injection) for mice injected with NAC (IP, 10 mg/kg in 25 μL of PBS, pH 7–8) or PBS (IP, 25 μL) two min prior to injection of a mixture of PCL-2 and D-cysteine (IP, 0.05 μmol each, in 50 μL of 1:1 DMSO:PBS) and a solution of H₂O₂ (IP, 1.5 μmol in 75 μL of PBS). (d) Total photon flux, 0–15 min post-injection, for mice injected with PCL-2 and H₂O₂ in the presence or absence of NAC. Statistical analyses were performed with a two-tailed Student’s t-test. *P < 0.05, **P < 0.01 (B: n = 4, D: n = 3) and error bars are ±SEM.