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Bmi-1 knockout delayed DNA damage repair in MCF-7 cell line. (A) Graph showing γH2AX foci in Bmi-1-depleted MCF-7 cells vs. control 5 min later after irradiation. Original magnification, ×600. (B) γH2AX foci positive cells after different dose irradiation. Mean ± SD of three experiments are reported. (C) γH2AX foci positive cells at different time point after 5 Gy irradiation between MCF-7-control cells and MCF-7-knockout cells. *P<0.001; t-test.
