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Bmi-1 depletion increased sub-G1 population. (A) Analysis of cell cycle distribution by flow cytometry. (B) Bmi-1 knockdown decreases mitochondrial membrane potential. Healthy mitochondria when it aggregates signal from JC-1 is depicted in red; when mitochondrial membrane potential collapses, signal corresponds to the green. Original magnification, ×400. (C) Quantitative results of fluorescence ratio (580 nm/530 nm).
