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Semiquantitative RT-PCR with primers designed against sorghum gene Sb07g023730 showing a 564-bp fragment in cDNA extracted from leaves and internodes of ICMP 451 (D2D2) (lane a) and no/a weak fragment of the same size in cDNA extracted from leaves and internodes of Tift 23DB (d2d2) (lane b). Lane c shows the 841-bp fragment obtained using genomic DNA from the tall inbred ICMP 451. Primers homologous to an actin gene were used as an internal control.
