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. 2012 Aug 8;28(4):1167–1175. doi: 10.3892/or.2012.1961

Figure 2.

Figure 2

Huaier extract induced apoptosis and caused cell cycle arrest in HUVECs. HUVECs were incubated with increasing concentrations of Huaier extract for up to 48 h. (A and C) Examples of flow cytometric data of HUVECs examined by PI-Annexin-V staining analysis after incubation with Huaier extract for 48 h. (B and D) Representative images of cell cycle analysis are shown, and the percentage of cells in each phase of the cell of each following treatment for 24 h is presented as a histogram, and all values were expressed as the means ± SD of 3 independent experiments conducted in triplicate. (E and F) Huaier extract induced the accumulation of p21. The bar graphs represent the relative density of p21/actin. Con, control. *P<0.05; **P<0.01; #P<0.05; ##P<0.01.