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. 2012 Aug 8;28(4):1167–1175. doi: 10.3892/or.2012.1961

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Copyright © 2012, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Effect of Huaier extract on the cell migration of HUVECs. (A) Confluent monolayers of HUVECs on a 12-well plate were wounded using a pipette tip and treated with Huaier extract or the vehicle. The images of wound closure were captured under a phase-contrast microscope after 48 h. (B) The migration inhibition is presented as distances between the 2 edges of the scratch. (C) Chemotaxis assay of HUVECs, which shows the inhibitory effect of Huaier extract on cell migration following 24 h of treatment. The cells successfully migrated to the lower surface of the insert. (D) The cell numbers decreased in a dose-dependent manner following treatment with Huaier extract. In the histograms, all values are expressed as the means ± SD. ^*P<0.05; ^**P<0.01 as compared with the vehicle.