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Huaier extract regulated multiple molecules in HUVECs. (A) After HUVECs were treated with various concentrations of Huaier extract, the expression levels of phosphorylated (P)-ERK and ERK were detected after 2 h, while the expression of VEGF was analyzed after 24 h by immunoblotting. β-actin was used as the loading control. (B) The bar graphs represent the mean relative densities ± SD of 3 independent experiments. Con, control. *P<0.05; **P<0.01.
