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H&E, CD34 and TUNEL staining of tumor tissues. 4T1 xenograft tissues were removed and cut into 4-μm thick slices. Representative images of (A) H&E staining showing the necrotic areas, (B) CD34 stainig, as an indicator of tumor microvessel density, and (C) TUNEL staining, where TUNEL-positive cells indicate apoptosis. The sites of hemorrhagic necrosis are indicated by arrows.
