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. 2011 Dec 6;27(4):1213–1218. doi: 10.3892/or.2011.1579

Figure 4.

Figure 4

The Akt pathway is involved in synthetic oligodeoxynucleotides (ODN) with the CpG-motif (CpG ODN)-mediated anti-apoptosis through tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) expression. (A) Cells were serum starved for 12 h, pretreated with 10 μl LY294002, and incubated with 3 μM CpG ODN for the indicated times. TRAIL mRNA levels were assessed by real-time PCR. (B) Cells were changed to serum free medium, pretreated with 10 μM LY294002, and incubated with 3 μM CpG ODN for 12 h. Cells were stained with propidium iodide (PI), and analyzed for the induction of apoptosis by flow cytometry. Each data point represents the mean ± SD of four independent experiments (*P<0.05 and **P<0.01). (C) Cells were transiently transfected with a control vector (pEGFP) or cDNA encoding wild-type Akt. Akt expression was assessed by Western blotting using anti-Akt antibody. Vector or Akt overexpressing cells were changed to serum-free medium and treated with 3 μM CpG ODN for 12 h, stained with PI, and analyzed for the induction of apoptosis by flow cytometry. Each data point represents the mean ± SD of four independent experiments (*P<0.05).