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Deguelin-inhibited DNA damage and repair gene expression in NCI-H640 cells were determined by real-time PCR. Total RNA was extracted from the NCI-H640 cells after treatment with 0, 50 and 250 nM deguelin for 24 h, and RNA samples were reverse-transcribed for real-time PCR as described in Materials and methods. The ratios of BRCA1, DNA-PK, MGMT, p53, ATM and ATR mRNA/GAPDH are shown and data represent the mean ± SD of three experiments. *p<0.05 was considered significant.
