Table I.
Identification of secreted proteins picked from spots whose expression was changed by nano-HPLC-ESI-TOF-MS/MS.
| Codea | Protein | Entry nameb | Scorec | M.W.d | pIe | Peptide sequencef | Amino acid numberg | Cover (%)h | Ratioi | P-valuej |
|---|---|---|---|---|---|---|---|---|---|---|
| 532 | Lactotransferrin precursor | TRFL | 75 | 78132 | 8.50 | LCAGTGENK | 191–199 | 4 | 0.41 | 0.033 |
| DLLFK | 316–320 | |||||||||
| DSAIGFSR | 321–328 | |||||||||
| CGLVPVAENYK | 424–435 | |||||||||
| YYGYTGAFR | 544–552 | |||||||||
| Serotransferrin precursor | TREF | 45 | 77000 | 6.81 | CLKDGADVAFUK | 213–225 | 4 | 0.44 | 0.005 | |
| DLLFK | 311–315 | |||||||||
| GDVAVK | 547–553 | |||||||||
| DLLFR | 647–651 | |||||||||
| 542 | Lactotransferrin precursor | TRFL | 44 | 78132 | 8.50 | LCAGTGENK | 191–199 | 4 | 0.44 | 0.005 |
| DLLFK | 316–320 | |||||||||
| CGLVPVAENYK | 424–435 | |||||||||
| YYGYTGAFR | 544–552 | |||||||||
| 742 | Protein S100-A8 | S10A8 | 259 | 10828 | 6.51 | LLETECPOYIR | 37–47 | 11 | 0.45 | 0.033 |
| 820 | Vitamin D binding protein precursor | VTDB | 46 | 52929 | 5.40 | LCDNLSTK | 285–292 | 1 | 0.45 | 0.028 |
| 1552 | 14-3-3 protein σ | 1433S | 285 | 27577 | 4.68 | ASLIQK | 4–9 | 31 | 2.87 | 0.005 |
| LAEQAER | 12–18 | |||||||||
| YEDMAAFMK | 19–27 | |||||||||
| SNEEGSEEKGPEVR | 69–82 | |||||||||
| EKVETELQGVCDTVLGLLDSHLIKk | 86–109 | |||||||||
| EKVETELQGVCDTVLGLLDSHLIKl | 86–109 | |||||||||
| VETELQGVCDTVLGLLDSHLIK | 88–129 | |||||||||
| MKGDYYR | 123–129 | |||||||||
| DSTLIMQLLR | 215–224 | |||||||||
| Protein S100-A9 | S10A9 | 259 | 13234 | 5.71 | MSQLER | 5–10 | 64 | 2.87 | 0.005 | |
| NIETINTFHQYSVK | 11–25 | |||||||||
| LGHPDTLNQEFK | 26–38 | |||||||||
| LGHPDTLNQEFKELVR | 26–42 | |||||||||
| DLQNFLK | 44–50 | |||||||||
| VIEHIMEDLDTNADK | 58–72 | |||||||||
| QLSFEEFIMLMAR | 73–85 | |||||||||
| Galectin-7 | LEG7 | 41 | 15066 | 7.03 | SSLPEGIRPGTVLR | 8–21 | 29 | 2.87 | 0.005 | |
| LDTSEVVFNSK | 55–65 | |||||||||
| GPGVPFQR | 76–73 | |||||||||
| HRLPLAR | 112–118 | |||||||||
| 1608 | Protein S100-A9 | S10A9 | 45 | 13234 | 5.71 | NIETINTFHQYSVK | 11–25 | 34 | 1.20 | 0.035 |
| LGHPDTLNQEFKELVR | 26–42 | |||||||||
| DLQNFLK | 44–50 |
a
Spot numbers correspond to those included in the 2-D image (Fig. 2. Spot 820 is not shown).
b
Swiss-Prot entry name;
c
confidence level for identification;
d
predicted molecular weight by amino acid sequence;
e
predicted pI by amino acid sequence;
f
identified amino acid sequence;
g
region of identified sequence;
h
coverage of identified region in total sequence;
i
average GEM treatment/control ratio;
j
P-value as calculated by DeCyder 6.5 software;
k
this peptide was measured for 2+ charged ions;
l
this peptide was measured for 3+ charged ions.