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(A) Inhibition of NAM N-oxide production by 100 μM chlorzoxazone (substrate NAM at 2 mM). (B) Inhibition of NAM N-oxide production by specific anti-CYP2E1 antibody (substrate NAM at 2 mM). (C) Dose-dependent increase in NAM N-oxide production by CYP2E1 overexpressing insect cell microsomes. Mean ± S.D. of triplicate determinations. *P < 0.05 by Student’s t test.
