Fig. 3.

Validation of Stc2 expression by qRT-PCR. qRT-PCR was performed on total RNA isolated from AhR^fx/fx primary hepatocytes infected with AdGFP or AdCreGFP for 24 hours or primary hepatocytes isolated from AhR^fx/fx and AhR CKO mice. The data presented for Stc2 represents the mean ± S.E.M. of three (AdGFP vs AdCreGFP) or two (AhR^fx/fx vs AhR CKO) independent experiments. Asterisk indicates statistical significance (P < 0.01) when compared to control infected hepatocytes. Quantification of mRNA expression is relative to ribosomal RNA 18S.