Fig. 7.
Adenoviral infection induces the UPR marker CHOP in primary hepatocytes. AhRfx/fx primary hepatocytes were infected with AdGFP or AdCreGFP or were allowed to remain uninfected for the indicated period, after which whole cell lysates were prepared. Western blot analysis was performed on AhR and CHOP. Actin was used as a loading control. (A) Quantification of CHOP protein expression 24 and 48 hours after adenoviral infection. The data presented for CHOP represents the mean ± S.E.M. of three independent experiments. Asterisk or number sign indicates statistical significance of P < 0.01 or P < 0.001, respectively, when compared with uninfected samples. (B) Representative Western blot showing loss of AhR and CHOP induction after infection with the adenovirus. (C) Induction of CHOP in murine immortalized hepatocyte AML-12 cells after infection with AdGFP and AdRFP.