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Analysis of HTS data: efficacy of hits at human GPR35. A distribution of agonist activity recorded in the 100,000 compound library HTS is shown. Compound activity at 10 µM using a human GPR35 PathHunter β-arrestin recruitment assay is shown both as hit frequency, binned as % agonist activity versus zaprinast, with number of hits for each % activity indicated above each bar. The pie chart shows the percentage of hits within each % activity group.
