Fig. 5.

Effect of pharmacological inhibition of mCA on high glucose-induced lipid peroxidation in IPCs. After exposure to normal glucose (NG, 5.7 mM), high glucose (HG, 40.7 mM), or high glucose with mCA inhibitors ethoxyzolamide or topiramate for 5 days. The HNE levels in IPCs were measured by immunoblotting. High glucose-induced significant increase in HNE (A and C) was almost completely reversed upon treatment with ethoxyzolamide (A) and topiramate (C) at both concentrations (10 and 100 µM) of the inhibitors used. (B) and (D) are the representative immunoblots of protein-bound HNE. The values are expressed as mean ± S.E.M. (n = 3). The graph is representative of three independent experiments. *P < 0.01; **P < 0.001; ***P < 0.0001. ETZ, ethoxyzolamide; HG, high glucose; NG, normal glucose; TOP, topiramate.