Fig. 1.

Chloroquine treatment does not affect clonogenic survival in 4T1 cells treated with radiation. 4T1 Cells were irradiated at 2, 5, or 10 Gy +/− CQ (10, 25, or 30 μM) and (A) evaluated for autophagy induction by Western blotting for LC3 or (B) allowed to recover for 7 days, when clonogenic growth was assessed. Graphs in (A) show density analysis of the mean ± S.E. of three independent experiments. *Different from untreated control; ^#different from control + CQ (P < 0.05). Graph in (B) shows quantification of clonogenic assays, where control was normalized to 100%. Graph shows mean ± S.E. of three independent experiments performed in triplicate. No statistical differences were found between untreated and CQ treated samples (P < 0.05).