Fig. 2.

Atg12 knockdown does not affect clonogenic survival in 4T1 cells treated with radiation. (A) 4T1 cells expressing an Atg12 or nonsilencing shRNA were treated with doxycycline (doxy) at the indicated concentrations for 72 hours and tested for Atg12 knockdown. (B) Autophagic flux measured by LC3II Western blot was evaluated in Atg12 shRNA-expressing cells treated ± irradiation, 1 μM doxycycline, or 10 μM chloroquine. (C) Survival was assessed as clonogenic growth of cells treated ± doxycycline 1 μg/ml, exposed to radiation (Rad) and allowed to recover for 7 days. Graphs show mean ± S.E. of three independent experiments performed in triplicate. Graphs in (B) show density analysis of the mean ± S.E. of three independent experiments. *Different from untreated control; ^#different from same treatment without doxycycline (P < 0.05).