Fig. 5.

Flow cytometry data for 6DBF7, DB16, and DB21 competitive binding to splenocytes. Multicolor flow cytometry was used to assess the effects of 6DBF7, DB16, and DB21 on FITC-gal-1 binding to splenocytes [CD4⁺ (A) and CD8⁺ (B) leukocytes and CD31⁺ (C) endothelial cells] derived from gal-1^−/− mice. Binding of 0.1 µM FITC-gal-1 to these cells was assessed as a function of concentration of 6DBF7, DB16, or DB21. Experiments were performed on an LSR II flow cytometer (BD Biosciences) and data were analyzed using Flowjo software (Tree Star, Inc.).