TABLE 1.

DBF-based compounds

Solubility was assessed at 5, 20, 50, and 100 µM compound in 20 mM Kphos buffer, pH 7.0, with 1% TFE, 1% glycerol, 2% NP40, and 150 mM NaCl. Solubilities are reported as >100 µM or <50 µM, depending upon whether a given compound followed Beer’s law up to 100 µM or only up to 50 µM, respectively. Proliferation of endothelial cells is reported in terms of IC₅₀ values (µM) for each compound. Bold letters indicate amino acids in the sequence that have been modified/changed.

Name	Sequence	Solubility	IC50
		µM	µM
Anginex	ANIKLSVQMKLFKRHLKWKIIVKLND	>100	4
6DBF7	SVQMKL-[DBF]-IIVKLND	<50	22
Single alanine substitution of 6DBF7
DB1	AVQMKL-[DBF]-IIVKLND	>100	19
DB2	SAQMKL-[DBF]-IIVKLND	<50	64
DB3	SVAMKL-[DBF]-IIVKLND	<50	28
DB4	SVQAKL-[DBF]-IIVKLND	>100	13
DB5	SVQMAL-[DBF]-IIVKLND	>100	202
DB6	SVQMKA-[DBF]-IIVKLND	>100	27
DB7	SVQMKL-[DBF]-AIVKLND	<50	19
DB8	SVQMKL-[DBF]-IAVKLND	>100	29
DB9	SVQMKL-[DBF]-IIAKLND	>100	31
DB10	SVQMKL-[DBF]-IIVALND	>100	74
DB11	SVQMKL-[DBF]-IIVKAND	<50	28
DB12	SVQMKL-[DBF]-IIVKLAD	<50	22
DB13	SVQMKL-[DBF]-IIVKLNA	<50	10
Double alanine substitution of 6DBF7
DB14	SVQAKL-[DBF]-IIVKLNA	>100	8
DB15	SVQAKL-[DBF]-AIVKLND	<50	9
DB16	SVQMKL-[DBF]-AIVKLNA	>100	5
Triple alanine substitution of 6DBF7
DB17	SVQAKL-[DBF]-AIVKLNA	<50	9
Norleucine substitution for Lys in 6DBF7
DB18	SVQMNleL-[DBF]-IIVKLNA	<50	9
DB30	SVQMKL-[DBF]- NleIVKLNA	<50	3
DB19	SVQMKL-[DBF]-IIVNleLNA	<50	15
DB29	SVQMNleL-[DBF]-IIVNleLNA	<50	20
Norvaline substitution for Val, Met, Leu, or Ile (branched to linear alkyl) in 6DBF7
DB25	SNvaQMKL-[DBF]-IIVKLNA	>100	10
DB21	SVQNvaKL-[DBF]-IIVKLNA	>100	3
DB22	SVQMKNva-[DBF]-IIVKLNA	<50	4
DB23	SVQMKL-[DBF]- NvaIVKLNA	<50	3
DB26	SVQMKL-[DBF]-IIVKNvaNA	<50	5
DB24	SVQMKL-[DBF]-IINvaKLNA	<50	3
DB27	SVQMKL-[DBF]-IIVKLNNva	<50	4
DB28	SVQNvaKNva-[DBF]- NvaINvaKLNA	<50	5