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Intracellular AMF expression of the HuO9 osteosarcoma cell line under hyperthermia with or without HSP inhibitors. Similar to mRNA, intracellular AMF was reduced by hyperthermia and recovered by the HSP27 inhibitor. Representative results of 3 different experiments are shown. a, control; b, hyperthermia; c, hyperthermia + 17-AAG; d, hyperthermia + KNK437; e, hyperthermia + KRIBB-III.
