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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Anal Chem. 2012 May 3;84(10):4535–4543. doi: 10.1021/ac300510t

Table 1.

IsoQuant peptide quantitation results from LC-MS/MS analysis of ABRF sPRG09 quantitative proteomics standard.

Protein UniProt
ID
Peptide sequence Expected
ratio
ABRF
consensus
ratio
Experimental
ratio
SD CV
(%)
% of
consensus
ratio
Absolute
deviation
(%)
ALBU_HUMAN LVNEVTEFAK 1 1 1.01 0.02 2.4 100.92 0.92
SLHTLFGDK 1.06 1.09 0.03 2.4 102.37 2.37
AEFAEVSK 1.03 1.07 0.05 5.1 103.77 3.77
NQO2_HUMAN NVAVDELSR 1 0.93 0.92 0.03 3.1 99.28 0.72
PRDX1_HUMAN DISLSDYK 0.1 0.1 0.10 0.01 6.5 103.24 3.24
ADEGISFR 0.11 0.10 0.01 10.1 89.37 10.63
GLFIIDDK 0.1 0.11 0.01 4.6 113.56 13.56
PILVQAFQFTDK 0.12 0.11 0.01 4.9 95.36 4.64
SYHC_HUMAN AALEELVK 5 3.73 3.92 0.11 2.9 104.99 4.99
DQGGELLSLR 3.5 3.03 0.40 13.2 86.53 13.47
GLAPEVADR 4.01 4.23 0.09 2.1 105.45 5.45
IFSIVEQR 4.24 4.10 0.48 11.8 96.63 3.37
UBIQ_HUMAN TITLEVEPSDTIENVK 10 8.28 9.09 0.99 10.9 109.74 9.74
TLSDYNIQK 7.81 8.37 0.24 2.9 107.11 7.11
ESTLHLVLR 10.7 11.96 0.82 6.8 111.77 11.77
Median - - - - - - 103.24 4.99