Fig. 5.

Selective mRNA markers of 17 dpc mouse pelvic urethra lamina propria, muscularis mucosa, submucosa, and muscularis propria. Near mid-sagittal sections (50 μm) of 17 dpc male and female LUT were stained by ISH to visualize mRNA expression (purple) of (A-B) the lamina propria marker snail homolog 1 (Snai1), (C-D) the lamina propria and submucosa marker forkhead box f1a (Foxf1a), and (E-F) the muscularis mucosa and muscularis propria marker actin alpha 2 (Acta2). Sections were then stained by immunofluorescence with an anti-smooth muscle actin alpha 2 (ACTA2) antibody that recognizes muscularis mucosa and muscularis propria (green) and an anti-cadherin 1 (CDH1) antibody that recognizes all urothelium (red). Inset images are magnified sections of mesenchyme that reveal the lamina propria (lp), muscularis mucosa (mm), submucosa (sm), and muscularis propria (mp) compartments. Staining patterns are representative of three males and three females. Abbreviations used are BL: bladder, ED: ejaculatory duct, LV: lower vagina, SV: seminal vesicle, UV: upper vagina. All images are of the same magnification.