Table 5. Reproducibility of Contributions for Disaccharide Standards from MS2 and MS3 Spectra.
% Ion Contribution | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
CID | Dimer | m/z | 1 | 2 | 3 | 4 | 5 | 6 | Av | SD |
MS2 | ΔUA2S-GlcNAc | 237.0 | 22.84 | 21.03 | 22.29 | 21.03 | 21.61 | 21.84 | 21.77 | 0.71 |
357.1 | 1.92 | 2.13 | 2.87 | 2.03 | 2.10 | 2.11 | 2.19 | 0.34 | ||
ΔUA-GlcNAc6S | 237.0 | 0.02 | 0.02 | 0.01 | 0.02 | 0.02 | 0.02 | 0.02 | 0.00 | |
357.1 | 23.07 | 23.60 | 23.31 | 23.57 | 23.32 | 24.06 | 23.49 | 0.34 | ||
MS3 | ΔUA2S-GlcNS | 297.1 | 29.60 | 30.21 | 31.08 | 30.40 | 29.12 | 31.04 | 30.24 | 0.78 |
339.1 | 9.38 | 10.11 | 9.30 | 11.03 | 8.88 | 9.49 | 9.70 | 0.76 | ||
ΔUA-GlcNS6S | 297.1 | 0.00 | 0.02 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.01 | |
339.1 | 62.29 | 63.41 | 63.24 | 63.10 | 62.65 | 62.47 | 62.86 | 0.45 |