Fig. 2.

FTIRI color maps and histogram distributions (70 bins for clarity) of the mineral/matrix ratio (phosphate/amide I peak area ratio) in cortical bone tissue. fro/fro mice were created on the FRA/Pas background and oim/oim on the C57Bl6 background. Graphs on the right show the mean (top) and widths (bottom) of the distributions of each genotype normalized by its respective wild type control for both cortical and trabecular tissues. The line indicates the level of the wild types for each parameter. A star above a bar indicates that a significant difference was found between mutant and wild type by performing a general linear model on the raw data. To compare between mutant genotypes and ages, the mutant values were normalized by the wild type values and then a GLM was performed. A bracket indicates significant differences that were found between the groups under the ends of the bracket. The fro/fro cortical tissue has significantly less mineral that was less heterogeneously distributed than WT and oim/oim tissues at 3 months, but both the mineral content and heterogeneity are normal by 6 months. The oim/oim tissue has higher mineral content than WT and remains constant over time.