Figure 3.

Two genotypes were identified at BAG-1 condon 324 in 142 patients with newly diagnosed advanced NSCLC (IIIB–IV stage). (A) PCR amplification product of BAG-1 DNA (861 bp). Marker, DL1000 DNA marker. (B) PCR products were digested by HpyCH4III restriction enzyme and separated on a 3% agarose gel. Marker, DNA marker 100 bp. (C) DNA sequencing of the PCR products. (D) Wave curves of DNA sequencing. Note that there was one single nucleotide mutation in the boxed site. Y stands for the C, T bases.