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ACS combination induces apoptosis in pancreatic cancer cells. MIA PaCa-2 cells were treated with aspirin SLN (ASP; 25 μM), curcumin SLN (CUR; 2.5 μM) and free sulforaphane (SFN; 5 μM) or in combination ACS (ASP+CUR+SFN) for 48 h. At the end of treatment, adherent and non-adherent cells were collected and stained with Annexin V-PI kit. Stained cells were then analyzed by flow cytometric analysis. The ACS chemopreventive combination demonstrated ∼61% apoptosis in MIA PaCa-2 cells.
