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FACS analysis of cell cycle and apoptosis.a
| CTRL (%) | VIII (%) | XII (%) | |
|---|---|---|---|
| G0/G1 phase | |||
| 0 h | 90 | 94 | 94 |
| 24 h | 93 | 90 | 87 |
| S phase | |||
| 0 h | 0.7 | 0.8 | 0.7 |
| 24 h | 0.1 | 0.9 | 0.7 |
| G2/M phase | |||
| 0 h | 1.3 | 2.3 | 2.5 |
| 24 h | 3.6 | 1.4 | 2.1 |
| h-apoptosis | |||
| 0 h | 6.5 | 2.1 | 1.6 |
| 24 h | 1.4 | 6.6 | 7.3 |
For detection and quantification of cell cycle distribution, samples containing 5×105 cells were harvested by centrifugation, fixed in cold ethanol and subjected to propidium iodide (PI) staining.
