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Effects of EGF on AR/ER/Src association. UMUC3 cells were cultured for 24 h in the presence of ethanol (mock) or 100 ng/ml EGF. Cell lysates were immunoprecipitated with anti-AR antibody or normal rabbit IgG and were then immunoblotted for AR (110 kDa), ERβ (56 kDa), or Src (60 kDa), as indicated.
