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. 2012 Oct 17;41(6):2021–2028. doi: 10.3892/ijo.2012.1671

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Copyright © 2012, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Effect of CD82 on the expression of Frizzled (Fzd) proteins. Whole cell lysates (200 μg) of h1299 cells [h1299/zeo (zeo), h1299/CD82 (CD82), h1299/CD82-sh.control (sh.cont), h1299/CD82-sh.CD82 (sh.CD82)] were resolved by 7.5% SDS-PAGE and analysed by immunoblotting with the anti-Fzd antibodies indicated in the figure. The same blots were re-blotted with an anti-CD82 antibody and an anti-β-actin as loading control. Experiments were performed in triplicate and the most representative data are shown.