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. 2012 Oct 17;41(6):2021–2028. doi: 10.3892/ijo.2012.1671

Figure 4.

Figure 4

Effect of CD82 on the expression of GSK-3β and CK1α protein and mRNA. (A) Whole cell lysates (200 μg) of h1299 cells [h1299/zeo (zeo), h1299/CD82 (CD82), h1299/CD82-sh.control (sh.cont), h1299/CD82-sh.CD82 (sh.CD82)] were resolved by 7.5% SDS-PAGE and analysed by immunoblotting with anti-GSK-3β and anti-CK1α antibodies. The same blots were re-blotted with an anti-CD82 antibody and an anti-β-actin as loading control. Experiments were performed in triplicate and the most representative data are shown. (B) Total RNA was isolated from h1299 cells and analysed by real-time RT-PCR. mRNA levels of GSK-3β were corrected relative to GAPDH mRNA. The asterisks in the figure indicate statistically significant difference (p<0.01) between the 2 values. Data are presented as the means ± SEM. (C) Total RNA was isolated from h1299 cells and analysed by real-time RT-PCR. mRNA levels of CK1α were corrected relative to GAPDH mRNA. Data are presented as the means ± SEM.