FIGURE 4:

Effect of modulating CDK activity in late meiosis on DNA replication. Meiosis was induced from G2, using cdc2-as spo4Δ strains, by Pat1 inactivation. Cell DNA contents, determined by flow cytometry, are shown. Here +Cdc18, Cdt1 indicates strains expressing Cdc18 and Cdt1 at physiological levels in late meiosis; otherwise cells were wt for Cdc18 and Cdt1 expression. The pink bar indicates that a pulse of 1NM-PP1 was applied at 6.75 h after meiotic induction and washed out 1 h later. The blue bar indicates that the temperature was reduced after meiotic induction (34°C, 0–3.5 h; 32°C, 3.5–5 h; 30°C 5–6.5 h; 27°C 6.5–10 h). The timing of events in meiosis induced from G2 is given in Supplemental Figure S4. Strains used were (A, B, E, F) P3028 and (C, D) P3027.