Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 1;24(5):617–632. doi: 10.1091/mbc.E12-07-0530

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Romero et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 7: — NIS, GM130, and Rab1b increase after TSH stimulation in FRTL5 cells. Left panels, immunofluorescence analysis in basal (−TSH) or stimulated (+TSH) situations; images for each marker were acquired under the same setting conditions. Right panels, flow cytometry analysis to measure fluorescence intensity to detect NIS, GM130, and Rab1b using a secondary antibody labeled with Alexa Fluor 647. Histograms represent profiles in basal (−TSH, dark gray) or stimulated (+TSH, black) conditions for the proteins indicated on the left panel. A total of 5 × 10⁵ cells were analyzed in each situation. Controls (light gray) were performed by incubating cells with only secondary antibody. The geometric mean fluorescence intensity value is indicated on top of each histogram.