FIGURE 1:

The formation of EGFP-CFTR–induced ERACs requires COPII function. (A) Wild-type (ANY21) cells containing the EGFP-CFTR expression plasmid were grown to mid–log phase at 23ºC and incubated with copper-containing medium for 2 h to induce expression, followed by observation with confocal microscopy. (B) Wild-type and sec12-4, sec23-1, sec13-1, sec16-2, sar1D32G, sec18-1, and sec17-1 strains were grown to mid–log phase and induced with copper at 23ºC (top) or 37ºC (bottom) for 2 h to induce EGFP-CFTR expression. ERAC formation is quantified using the three categories that were defined in A, and the data are presented as the percentage of cells expressing EGFP-CFTR. The experiments were repeated at least three times, and >100 cells were analyzed for each condition.