Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 1;24(5):659–667. doi: 10.1091/mbc.E12-10-0721

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Losón et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 3: — Knockdown of MiD49 or MiD51 causes mitochondrial elongation and enhances the Fis1-null, Mff-null, and Fis1/Mff-null phenotypes. (A) Mitochondrial morphology in wild-type MEFs treated with control siRNA, siRNA against MiD49, siRNA against MiD51, or both. Scale bar, 10 μm. Insets are magnified images of the boxed regions. Scale bar, 5 μm. Mitochondria were highlighted by expression of Cox8-DsRed. (B) Western blot of cell lysates containing single and double knockdown of the MiDs. SOD2 is a loading control. (C) Scoring of mitochondrial network morphologies for knockdown experiments in wild-type, Fis1-null, Mff-null, and Fis1/Mff-null cells. Data were obtained from three independent experiments, with 100 cells scored per experiment. Error bars, SEM. C, collapsed; L, long; N, net-like; S, short.