Figure 1.

Effects of budesonide on lung infection in mice exposed to P. aeruginosa. A) Animal model. Mice were sensitized by intraperitoneal injection with ovalbumin (OVA) in aluminum hydroxide on days 1 and 8. From days 15 through 22, mice were intranasally challenged with OVA. OVA-challenged mice were treated with inhaled budesonide on days 23 through 30. On day 31, budesonide-treated mice were infected intranasally with 1 x 10⁷ CFU ml^-1P. aeruginosa. Mice were killed after 24 h of P. aeruginosa infection. B) Lung samples were stained with hematoxylin and eosin (HE) (original magnification ×200). OVA/Bud mice had less extensive infiltration of inflammatory cells in the airways than OVA/No mice. However, OVA/Bud/P.a mice developed more inflammation and cellular infiltration than OVA/P.a mice.