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Validation of the CK8/CK18 complex as the K94 antigen using siRNA to CK8 or CK18. (A) RT-PCR analysis of CK8 or CK18 expression in MCF-7 cells treated with siRNA against CK8 or CK18. (B) FACS and (C) western blot analysis of MCF-7 cells treated with siRNA targeting CK8 or CK18 and stained with K94 antibody. Western blots were also probed with anti-CK8 or CK18 antibody. (D) Immunofluorescence staining of HepG2 and MCF-7 cells with K94 antibody.
