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. 2012 Oct 26;42(1):65–74. doi: 10.3892/ijo.2012.1679

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Copyright © 2013, Spandidos Publications

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Figure 5. — Human serum ELISA for the detection of autoantibody against CK8/CK18 complexes. (A) Detection of autoantibody against CK8/CK18 complexes in HCC patients’ sera by K94p1 phage ELISA. (B) Detection of CA15.3 in breast cancer patients’ sera using commercialized kits (eBioscience, San Diego, CA). (C) Detection of autoantibody against CK8/CK18 complexes in breast cancer patients’ sera by K94p1 phage ELISA. The specific binding of autoantibody against K94p1 phage was expressed as the difference between the OD value of K94p1 ELISA and that of empty phage (Eph) ELISA. These experiments were repeated three times and a representative result is shown. (D) ROC curve of K94p1 phage ELISA in Fig. 5C.