Figure 6.

Effect of caspase inhibition upon in vitro sensitivity to cisplatin. (A) JU77 (B) LO68 (C) ONE58. Cells were cultured in the presence of cisplatin (0–20 μg/ml) for 24 h following exposure to caspase inhibitors. Cell viability was determined by MTT assay. Results are mean ± SD of three independent treatments performed in triplicate. (D) Caspase inhibitors effectively block cisplatin induced caspase activation. Cells were incubated with a range of caspase inhibitors and exposed to cisplatin (0–20 μg/ml) for 24 h. Caspase activity was measured after 24 h and expressed as fold increase relative to untreated cells. Results are the mean ± SD of at least two independent treatments performed in triplicate.