Figure 1.

The enzymatically active form of Pf-calpain is identified. A) Schematic diagram of the full-length Pf-calpain and three recombinant Pf-calpain constructs. Cys 1035, His 1371, and Asn 1391 were indicated in catalytic subdomain IIa and IIb, respectively. The predicted histidine (His 1179) and asparagine (Asn 1195) residues were shown in red. These residues were further analysed. Black boxes indicate the recognition sites of anti-Pfcalpain antibodies. Left, anti-PfCapnA antibody; Right, anti-PfCapnB antibody. B) Western blot of purified recombinant Pf-calpains using an anti-Pf-calpain antibodies. C) Calpain enzymatic activity measured by the fluorogenic substrate assay. D) Calpain enzymatic activity measured by gelatin zymography.