Skip to main content
. 2013 Feb 27;8(2):e57562. doi: 10.1371/journal.pone.0057562

Figure 4. Effect of MCL-1 overexpression on cell viability.

Figure 4

(A) MCL-1 overexpression partially blocks PGs cytotoxicity. SK-MEL-5 cells were transfected with 1 µg of pTOPOMCL1 plasmid and, after 20 h, cells were treated with PGs (2 and 20 µM, respectively) for an addittional 24 h. Then cell viability was assessed by the MTT assay. (B) Analysis of MCL-1 protein levels. After MCL1 overexpression and PGs treatment, MCL-1 protein levels were analyzed by Immunoblot. Vinculin was used as a loading control.