Figure 4.

Cotransfected full-length β3 subunits bind homophilically. A–C) HEK293 cells were cotransfected with plasmids expressing β3-wt-EGFP and β3-wt-Myc (A); transfected with β3-wt-EGFP alone (B); separately transfected with plasmids expressing β3-wt-EGFP and β3-wt-Myc, then cell lysates were mixed together prior to immunoprecipitation (C); or separately transfected with plasmids expressing β3-wt-EGFP and β3-wt-Myc, then cells were cocultured for 2 d under conditions of 70–90% confluency prior to cell lysis (D). In each case, equal volumes of samples were immunoprecipitated with anti-Myc resin and blotted with antibodies as indicated. FT, flow through; W, wash; E, elution fraction; Mwt, molecular weight (mass).