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. 2013 Feb 27;8(2):e57582. doi: 10.1371/journal.pone.0057582

Figure 3. Anchorage-independent growth of chicken ovarian cancer (COVCAR) and normal ovarian surface epithelial (NOSE) cells.

Figure 3

COVCAR and NOSE cells were cultured in semi-solid media for 4 weeks as described in Materials and Methods section. A. NOSE cells remaining as single cells showing no detectable changes in morphology. B. COVCAR cells on day 6 of culture showing numerous projections on the surface and forming solid ball-like structure. C-D. Multiple colonies and a large sphere-like structure of COVCAR cells on day 11 of culture. E. Two acini composed of COVCAR cells on day 13 of culture. F. Proliferating COVCAR cells invading through agarose. G. A large sphere of COVCAR cells and several tube-like structures. Scale bars = 100 µm.