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. 2013 Feb 27;8(2):e57582. doi: 10.1371/journal.pone.0057582

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© 2013 Tiwari et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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RT-PCR analyses for expression of various cytoskeletal proteins, growth factors and receptors, protein/enzymes related to steroid hormone synthesis, gonadal hormone and hormone receptors in chicken ovarian cancer cell lines (C5, C6, C7, C11, C19) and normal ovarian surface epithelial cells (NOSE; n = 5 animals). Total RNA was extracted from cultured cells in passages 3–4 and treated with deoxyribonuclease-I. Approximately 250 ng of cDNA (+RT) was used as template to amplify the gene products. Contamination controls consisted of reverse transcribed RNA without reverse transcriptase (-RT). M- DNA size marker.