Figure 1. Schematic representation of the genome-wide method for RNA decay using transcriptional inhibitor-mediated global transcriptional arrest. Transcriptional inhibitors such as Actinomycin D (ActD), 5,6-dichloro-1–D-ribofuranosyl-benzimidazole (DRB) or α-amanitin (α-Am) are added to cells, collect the cells at sequential time points after addition of the transcriptional inhibitors, and extract total RNA (blue).