Figure 3. In vitro translation of CGC encoded arginine by a wheat germ extract. (A) Sequence of RT/PCR product from tRNA^Arg_ACG extracted from commercial wheat germ cell free translation extract. The anticodon is underlined. (B) SDS gel analysis of in vitro translation using wheat germ extract. Product formation was monitored by phosphorimager analysis of the incorporation of [³⁵S]-Met into EGFP in whose gene all arginine codons had been replaced by CGC. Lanes 1 and 2, EGFP protein (predicted, 26.9 kDa); 3, Flexi ^® Vector without an integrated gene sequence; 4, Luciferase positive control (predicted, 61 kDa); M, Protein Ladder. Samples and marker were run on the same gel. The unlabelled protein ladder was visualized optically and the separate images scaled to the identical size. The red molecular weight bands of 40, 70 and 260 kDa of the marker were not visible under the conditions of the image capture. (C) Fluorescence emission spectrum of the translation products EGFP (green), translation vector only (pink triangles), luciferase (purple) and no translation template (blue). Excitation was at 470 nm.