Table 1. Primers used for reverse transcription, PCR and sequencing.

tRNA	Reverse transcription	PCR	Sequencing
Soybean tRNAArgACG	1	1+3	7
Jack bean tRNAArgACG	1	1+3	7
Wheat tRNAArgACG	1	1+3	7
E. coli tRNAArgICG	1	1+4	1
Jack bean tRNASerIGA	1	1+5	8
Moss mitochondrial tRNAArgICG	1	2+6	7
E.coli tRNAArgACG gene		4+9	4
Primer	Sequence
1	M13 universal primer
2	ACGGCCAGTGCAGTGAGTGGCATG
3	TTTAATACGACTCACTATAGACTCCATGGCCCAATGGAT
4	TTTAATACGACTCACTATAGCATCCGTAGCTCAGCTGGA
5	TTTAATACGACTCACTATAGTGGACGTGCCGGAGTGGTTA
6	TTTAATACGACTCACTATAGTGCTTGTAGCTCAATCGGATA
7	TACAGGAAACAGCTATGACCCATCGTGATCCTTAGACTTCATACACTTACTTTAATACGACTCACTATA
8	ACGGCCAGTGCAGTGAGTGG
9	CCCTGCAGTGGTGCATCCGGGAGGATTCGA

The following combination of primers was used to characterize the tRNAs described, after ligation of an RNA tail to the 3' end.