Figure 4.

TALEN-induced mutations observed in F₁ larvae from seven G0 founders. Red letters or dashes indicate the identified mutations. Red and blue boxes in the wild-type (WT) sequences indicate the left and right recognition sites of the TALENs, respectively. Green boxes indicate the HaeIII cleavage site. The sizes of the insertions and deletions are shown to the right of each mutated sequence (−, deletions; +, insertions). The numbers on the right edge indicate the numbers of larvae carrying each mutated sequence among all sequenced larvae.