Figure 2.

Effects of Pols ζ and η on pol2-4 msh2Δ synthetic lethality. pol2Δ msh2Δ POL2–URA3 cells defective for Pol ζ (rev3Δ) or Pol η (rad30Δ) were transformed with POL2– or pol2-4–LEU2 plasmids, and 10-fold serial dilutions of isolated transformants were plated onto FOA-containing media as in Figure 1. For comparison, the POL2– and pol2-4–LEU2 plasmids were similarly shuffled into strains that were wild type (WT) for Pols ζ and η (REV3 RAD30) and either msh2Δ or MSH2 (WT MMR). The LEU2 plasmid with no POL2 or pol2-4 gene served as the vector-only control. Colony formation was assessed after incubation at 30° for 3 days. Neither rev3Δ nor rad30Δ rescued pol2-4 msh2Δ synthetic lethality.