Figure 6.

Spontaneous CAN1 mutations from pol2-4 and pol2-4,eex msh6Δ cells. The can1 genes from ∼50 independent Can^r mutants of each strain were PCR-amplified and sequenced. Mutations identified in different strains are color-coded according to the key at the bottom. Each base letter above the wild-type CAN1 sequence indicates an independent base substitution or frameshift (+ or −) mutation. Horizontal lines indicate CAN1 sequences involved in complex mutations, duplications, and deletions. The wild-type POL2 and POL2 msh6Δ spectra are from Herr et al. (2011a).